Coliform ChromoSelect Agar
SIAL/81938 - NutriSelect® Plus, suitable for microbiology
Synonym: Coliform Agar ChromoSelect
Product Type: Chemical
Selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms
| application(s) | agriculture bioburden testing environmental food and beverages water monitoring |
| microbiology | |
| composition | agar, 12 g/L |
| chromogenic mixture, 0.2 g/L | |
| dipotassium hydrogen phosphate, 3 g/L | |
| peptone, special, 3 g/L | |
| potassium dihydrogen phosphate, 1.7 g/L | |
| sodium chloride, 5 g/L | |
| sodium lauryl sulfate, 0.1 g/L | |
| sodium pyruvate, 1 g/L | |
| tryptophan, 1 g/L | |
| final pH | 6.8±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective and differential for Citrobacter spp. |
| selective and differential for Enterobacter spp. | |
| selective and differential for Escherichia coli | |
| selective and differential for Klebsiella spp. | |
| selective and differential for Salmonella spp. | |
| selective and differential for Shigella spp. | |
| selective and differential for coliforms | |
| selective and differential for enterobacteriaceae |
| Application: | Coliform ChromoSelect Agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms in water and food samples. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Coliform ChromoSelect Agar is a chromogenic mixture which contains two chromogenic substrates, Salmon-GAL and X-glucuronide. Coliforms produce an enzyme called β-D-galactosidase, which cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by Escherichia coli cleaves X-glucuronide. Escherichia coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction, thereby increasing detection reliability in combination with the two chromogens. To confirm Escherichia coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 27 g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. When a high number of gram-positive accompanying bacteria are expected, add 5 mg/l Novobiocin (Cat. No. 74675) before autoclaving of the medium. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |