Mac Conkey-Sorbitol ChromoSelect Agar
SIAL/83339 - suitable for microbiology, NutriSelect® Plus
Synonym: Mac Conkey-sorbitol Agar ChromoSelect; SMAC Agar ChromoSelect; Sorbitol MacConkey Agar ChromoSelect
MDL Number: MFCD00240806
Product Type: Chemical
| application(s) | agriculture environmental food and beverages |
| microbiology | |
| composition | agar, 13.5 g/L |
| B.C. indicator, 0.1 g/L | |
| bile salts mixture, 1.5 g/L | |
| casein enzymic hydrolysate, 17 g/L | |
| crystal violet, 0.001 g/L | |
| neutral red, 0.03 g/L | |
| proteose peptone, 3 g/L | |
| sodium chloride, 5 g/L | |
| sorbitol, 10 g/L | |
| final pH | 7.1±0.3 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective and differential for Escherichia coli |
| selective and differential for coliforms | |
| selective and differential for enterobacteriaceae | |
| selective and differential for enterovirulent E. coli (EEC) |
| Application: | Mac Conkey-Sorbitol ChromoSelect Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme β- |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 50.1g in 1 litre distilled water. Boil gently to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C. Mix well and pour into sterile petri plates. If desired add Tellurite-Cefixime Supplement to the molten and cooled medium (50°C) before pouring into sterile petri plates. |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| Risk Statements | 52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |