m-Endo Agar LES
SIAL/85766 - suitable for microbiology, NutriSelect® Plus
Synonym: Membrane Endo Agar LES
Product Type: Chemical
Used for the enumeration of coliforms in water
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 85766-500G-F
| application(s) | agriculture bioburden testing environmental food and beverages water monitoring |
| microbiology | |
| composition | agar, 15 g/L |
| casein hydrolysate, 3.7 g/L | |
| di-potassium hydrogen phosphate, 3.3 g/L | |
| lactose, 9.4 g/L | |
| pararosanilin (fuchsin), 0.8 g/L | |
| peptone from meat, 3.7 g/L | |
| potassium hydrogen phosphate, 1 g/L | |
| sodium chloride, 3.7 g/L | |
| sodium deoxycholate, 0.1 g/L | |
| sodium lauryl sulfate, 0.05 g/L | |
| sodium sulfite, 1.6 g/L | |
| tryptose, 7.5 g/L | |
| yeast extract, 1.2 g/L | |
| final pH | 7.2±0.2±25 °C |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective and differential for Escherichia coli |
| selective and differential for Proteus spp. | |
| selective and differential for Salmonella spp. | |
| selective and differential for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | m-Endo Agar LES can be used for the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration. This method is more reliable and precise than MPN multiple test. It follows the two step membrane filter procedure using Lauryl Sulfate Broth (Cat. No. 17349) as a preliminary enrichment, resulting in higher coliform counts. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 51 g in 1 litre of distilled water containing 20 ml of ethanol 96 %. Boil to dissolve completely. Do not autoclave! Cool to 45-50°C and dispense 4 ml amounts into 50-60 mm Petri dishes and allow to solidify. The agar should be at least 1.5mm depth. Do not expose plates to direct sunlight. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H350 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 45 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |