Eosin B
SIAL/861006 - certified by the Biological Stain Commission, Dye content 90 %
Synonym: 4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt; Acid Red 91; Eosin Scarlet; Eosin bluish; Saffrosine
CAS Number: 548-24-3
Empirical Formula (Hill Notation): C20H6Br2N2Na2O9
Molecular Weight: 624.06
EC Number: 208-943-1
MDL Number: MFCD00005041
Linear Formula: C20H6N2O9Br2Na2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 861006-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 861006-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 861006-10G
| λmax | 395 nm |
| 514 nm | |
| application(s) | diagnostic assay manufacturing hematology histology |
| composition | Dye content, 90% |
| form | powder |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C20H8Br2N2O9.2Na/c21-1 |
| InChI key | GYYTYUGGVBYJHE-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Na+].[Na+].[O-]C(=O)c1cc |
| solubility | H2O: 1 mg/mL, clear, red |
| storage temp. | room temp |
| Application: | • Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats. • It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections. • It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse. |
| Biochem/physiol Actions: | Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer’s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration. |
| Packaging: | 10, 25, 100 g in glass bottle |
| Suitability: | Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 45400; 45400 |
| UNSPSC | 12171500 |