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Toluidine Blue

SIAL/89640 - for microscopy (Hist., Vit.), 8.74% (ZN (THEORY))

Synonym: Tolonium chloride; Basic Blue 17

CAS Number: 6586-04-5
Empirical Formula (Hill Notation): C15H16ClN3S · 0.5ZnCl2
Molecular Weight: 373.97
MDL Number: MFCD03548293
Linear Formula: C15H16ClN3S · 0.5ZnCl2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-89640-5G 5 g
 $58.10
1/EA		 Add To Favorites
45-89640-25G 25 g
 $177.00
1/EA		 Add To Favorites

 

εmax ≥800 at 621-636 nm in water
application(s) diagnostic assay manufacturing
hematology
histology
assay 8.74% (ZN (THEORY))
color green to very dark green
form powder or crystals
grade for microscopy (Hist., Vit.)
InChI 1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
InChI key DAQXTSXITPUGRG-UHFFFAOYSA-L
Quality Level 200 
SMILES string [Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(C=C3Sc2cc1N)=[N+](C)C.Cc4cc5N=C6C=CC(C=C6Sc5cc4N)=[N+](C)C
solubility methanol: 0.01 g/10 mL, blue to very deep blue
storage temp. room temp
Application: Toluidine Blue is suitable for use in the following applications:
  1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
    •  in metachromatic staining of sulfated mucins and related materials
    • as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
    •  staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)
Biochem/physiol Actions: Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.
General description: Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity 8.74% (ZN (THEORY))
Storage Temp. room temp
Colour Index Number 52040
UNSPSC 12171500

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