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4-(Dimethylamino)azobenzene-4′-sulfonyl chloride

SIAL/89802 - for HPLC derivatization, LiChropur™, 98.0-102.0% (AT)

Synonym: 4-(4-Dimethylaminophenylazo)benzenesulfonyl chloride; DABS-Cl; Dabsyl chloride

CAS Number: 56512-49-3
Empirical Formula (Hill Notation): C14H14ClN3O2S
Molecular Weight: 323.80
EC Number: 260-235-1
MDL Number: MFCD00007444
Linear Formula: (CH3)2NC6H4N=NC6H4SO2Cl
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-89802-500MG 500 mg
 $161.00
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assay 98.0-102.0% (AT)
form crystals
grade for HPLC derivatization
InChI 1S/C14H14ClN3O2S/c1-18(2)13-7-3-11(4-8-13)16-17-12-5-9-14(10-6-12)21(15,19)20/h3-10H,1-2H3/b17-16+
InChI key VTVWTPGLLAELLI-WUKNDPDISA-N
mp 185 °C (dec.) (lit.)
quality LiChropur™
Quality Level 100 
SMILES string CN(C)c1ccc(cc1)N=Nc2ccc(cc2)S(Cl)(=O)=O
technique(s) HPLC: suitable
Application: 4-(Dimethylamino)azobenzene-4′-sulfonyl chloride may be used to derivatize biogenic amines, primary amino acids, melamine, and hydroxyproline and proline extracted from various biological samples prior to their determination using high performance liquid chromatography (HPLC) technique to increase their detection sensitivity.
General description: Amino groups are common to many biological compounds and many amines used in pharmaceuticals. Most of these compounds show weak absorption in direct HPLC analysis with UV-VIS detection. However, strongly absorbing derivatives of amines can be easily detected by HPLC with high sensitivity even when present at low concentrations. Primary and secondary amino groups are usually derivatized as aromatic derivatives by nucleophilic substitution reactions using reagents such as arylsulfonyl chlorides.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.
Legal Information: LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
Recommended products: Discover LiChropur™ reagents ideal for HPLC  or LC-MS  analysis
Purity 98.0-102.0% (AT)
mp 185 °C (dec.) (lit.)
UNSPSC 12352200

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