Dde biotin picolyl azide
SIAL/901991
Synonym: N-
CAS Number: 2055048-42-3
Empirical Formula (Hill Notation): C38H57N9O9S
Molecular Weight: 815.98
MDL Number: MFCD30458027
Linear Formula: C38H57N9O9S
Product Type: Chemical
| form | solid |
| InChI key | CYXSXYDLWXHYSI-JKMQWNNCSA |
| reaction suitability | reaction type: click chemistry |
| SMILES string | S1[C@@H]([C@@H]4NC(=O)N[C |
| storage temp. | −20°C |
| Application: | Dde biotin picolyl azide is a unique alkyne-reactive, cleavable biotin probe that allows for release of the captured biomolecules from streptavidin under mild conditions. This reagent contains a biotin moiety linked to a picolyl azide moiety through a spacer arm containing a hydrazine-cleavable Dde moiety. Captured biomolecules can be efficiently released, typically >90% with 2% hydrazine in aqueous media. Dde biotin picolyl azide incorporates a copper-chelating motif that dramatically accelerates the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction under conditions relevant to biomolecular labeling. Discovery of azides with an internal Cu(I)-chelating motif greatly enhances the utility of the CuAAC reaction for various applications. Some examples are monitoring the dynamic glycan biosynthesis in living organisms; sensitive detection of metabolically-labeled proteins, DNAs, and RNAs; and many other applications where rate acceleration and reduced cell toxicity are highly desirable. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352125 |