Desthiobiotin-PEG3-Azide
SIAL/902020
Synonym: (4S,5R)-N-
CAS Number: 1951424-99-9
Empirical Formula (Hill Notation): C18H34N6O5
Molecular Weight: 414.50
MDL Number: MFCD28334534
Linear Formula: C18H34N6O5
Product Type: Chemical
form | paste |
storage temp. | −20°C |
Application: | Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 51171641 |