Mueller Hinton Broth 2
SIAL/90922 - NutriSelect® Plus, suitable for microbiology, Cation-adjusted
Synonym: M-H 2 Broth; M-H 2 Broth, Mueller Hinton II Broth; Mueller Hinton II Broth
Product Type: Chemical
Intended for use in quantitative procedures for susceptibility testing
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 90922-500G
| application(s) | clinical testing food and beverages |
| microbiology | |
| color | light yellow |
| composition | acid hydrolysate of casein, 17.5 g/L |
| beef extract, 3 g/L | |
| starch, 1.5 g/L | |
| final pH | 7.3±0.2 (25 °C) |
| form | (homogeneous, hygroscopic powder) |
| manufacturer/tradename | NutriSelect® Plus |
| packaging | bottle of 500 g |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | nonselective for Enterococcus spp. |
| nonselective for Escherichia coli | |
| nonselective for Neisseria spp. | |
| nonselective for Pseudomonas spp. | |
| nonselective for Staphylococcus spp. | |
| nonselective for bacteria (General Media) | |
| nonselective for coliforms | |
| technique(s) | microbe id | susceptibility testing: suitable |
| microbiological culture: suitable |
| Application: | Mueller Hinton Broth 2 cation adjusted is intended for use in quantitative procedures for susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. It has been used for culturing Escherichia coli, Bacillus subtilis, Staphylococcus aureus and in the serial dilution of doxycycline. |
| Features and Benefits: | NutriSelect® Plus are powdered culture media offering• Quality control with growth promotion test according to industry standards • Quality assurance acc. to ISO 9001 • Cost-efficient powdered media |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Mueller Hinton Broth 2 is recommended for testing the most encountered aerobic and facultative anaerobic bacteria found in food and clinical material for antimicrobial susceptibility testing. The medium shows good batch-to-batch reproducibility. It is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.Casein acid hydrolysate and beef extract provide amino acids and other nitrogenous substances, minerals, vitamins, and carbon to support the growth of microorganisms. To determine the MIC values with Enterococcus faecalis and sulfamethoxazole-trimetho |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 22 g in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense and sterilize by autoclaving at 10-15 lbs pressure (115-121°C) for 10 minutes. DO NOT OVERHEAT. Mix well before pouring. Note: This medium is supplemented with appropriate salts to provide 20-25 mg/l of calcium and 10-12.5 mg/l of magnesium and as additionally required to suit performance parameters. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171504 |