Mueller Hinton Broth 2
SIAL/90922 - suitable for microbiology, Cation-adjusted, NutriSelect® Plus
Synonym: M-H 2 Broth; M-H 2 Broth, Mueller Hinton II Broth; Mueller Hinton II Broth
Product Type: Chemical
application(s) | clinical testing food and beverages |
microbiology | |
composition | acid hydrolysate of casein, 17.5 g/L |
beef extract, 3 g/L | |
starch, 1.5 g/L | |
final pH | 7.3±0.2 (25 °C) |
form | (homogeneous, hygroscopic powder) |
manufacturer/tradename | NutriSelect® Plus |
Quality Level | 200 ![]() |
shelf life | limited shelf life, expiry date on the label |
sterility | non-sterile |
suitability | nonselective for Enterococcus spp. |
nonselective for Escherichia coli | |
nonselective for Neisseria spp. | |
nonselective for Pseudomonas spp. | |
nonselective for Staphylococcus spp. | |
nonselective for bacteria (General Media) | |
nonselective for coliforms | |
technique(s) | microbe id | susceptibility testing: suitable |
microbiological culture: suitable |
Application: | Mueller Hinton Broth 2 cation adjusted is intended for use in quantitative procedures for susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. It has been used for culturing Escherichia coli, Bacillus subtilis, Staphylococcus aureus and in the serial dilution of doxycycline. |
Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
Preparation Note: | Suspend 22 g in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense and sterilize by autoclaving at 10-15 lbs pressure (115-121°C) for 10 minutes. DO NOT OVERHEAT. Mix well before pouring. Note: This medium is supplemented with appropriate salts to provide 20-25 mg/l of calcium and 10-12.5 mg/l of magnesium and as additionally required to suit performance parameters. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 41171504 |