Rappaport Vassiliadis medium (base), modified, semi-solid
SIAL/92322 - suitable for microbiology, NutriSelect® Plus
Synonym: MSRV medium (base); Modified Semisolid Rappaport Vassiliadis Medium (base)
Product Type: Chemical
Modified Semi-Solid Rappaport Vassiliadis medium for enrichment of Salmonellae.
| application(s) | environmental food and beverages sample preparation |
| microbiology | |
| composition | agar, 2.7 g/L |
| casein peptone, 4.59 g/L | |
| magnesium chloride, 10.93 g/L | |
| malachite green, 0.037 g/L | |
| mono-potassium phosphate, 1.47 g/L | |
| sodium chloride, 7.34 g/L | |
| tryptose, 4.59 g/L | |
| final pH | 5.2±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| suitability | selective for Salmonella spp. |
| technique(s) | microbiological culture: suitable |
| Application: | The modified Semi-Solid Rappaport Vassiliadis medium is formulated according De Smedt and et al. for enrichment of Salmonellae. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 31.6 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add 20 mg/L of Novobiocin (Cat. No. 74675). Mix well and pour into sterile petri plates. Keep plates in a fresh place to settle the gel (1 hour minimum) and handle with care because the medium is only semi-solid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41171606 |