Tris-EDTA buffer solution

SIAL/93283 - BioUltra, for molecular biology, pH 8.0

Synonym: TE buffer solution

MDL Number: MFCD00236359
Product Type: Chemical

λ	neat
cation traces	Al: ≤1 mg/kg
	Ba: ≤1 mg/kg
	Bi: ≤1 mg/kg
	Ca: ≤5 mg/kg
	Cd: ≤1 mg/kg
	Co: ≤1 mg/kg
	Cr: ≤1 mg/kg
	Cu: ≤1 mg/kg
	Fe: ≤1 mg/kg
	K: ≤20 mg/kg
	Li: ≤1 mg/kg
	Mg: ≤1 mg/kg
	Mn: ≤1 mg/kg
	Mo: ≤1 mg/kg
	Ni: ≤1 mg/kg
	Pb: ≤1 mg/kg
	Sr: ≤1 mg/kg
	Zn: ≤1 mg/kg
form	liquid
grade	for molecular biology
impurities	DNases, none detected
	insoluble matter, passes filter test
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key	VLEIUWBSEKKKFX-UHFFFAOYSA-N
pH	8.0±0.2 (25 °C)
product line	BioUltra
Quality Level	200
SMILES string	NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
UV absorption	λ: 260 nm A_max: 0.04
	λ: 280 nm A_max: 0.01

Application:	Tris-EDTA buffer solution has been used as a component in Tris-EDTA (TE) buffer to resuspend DNA pellets for total DNA extraction from herbage samples and single-stranded carrier DNA extraction from salmon sperm.
Biochem/physiol Actions:	Ethylenediaminetetraacetic acid (EDTA) and its salts are used in a variety of medical and pharmaceutical applications. It is a calcium chelator that also acts as an anticoagulant and a detoxicant in the case of heavy metal poisoning. EDTA is frequently used in analytical chemistry for complexometric titrations and several other applications. It is used as a food additive to bind metal ions due to its low toxicity.
Biochem/physiol Actions:	Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.
Features and Benefits:	• DNases, RNases, phosphatases, and protease-free • Insoluble matter, passes filter test
Other Notes:	This buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

12161700