Tris-EDTA buffer solution

SIAL/93283 - BioUltra, for molecular biology, pH 8.0

Synonym: TE buffer; TE buffer solution

MDL Number: MFCD00236359
Product Type: Chemical

λ	neat
application(s)	sample preparation
cation traces	Al: ≤1 mg/kg
	Ba: ≤1 mg/kg
	Bi: ≤1 mg/kg
	Ca: ≤5 mg/kg
	Cd: ≤1 mg/kg
	Co: ≤1 mg/kg
	Cr: ≤1 mg/kg
	Cu: ≤1 mg/kg
	Fe: ≤1 mg/kg
	K: ≤20 mg/kg
	Li: ≤1 mg/kg
	Mg: ≤1 mg/kg
	Mn: ≤1 mg/kg
	Mo: ≤1 mg/kg
	Ni: ≤1 mg/kg
	Pb: ≤1 mg/kg
	Sr: ≤1 mg/kg
	Zn: ≤1 mg/kg
form	liquid
grade	for molecular biology
impurities	DNases, none detected
	insoluble matter, passes filter test
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key	VLEIUWBSEKKKFX-UHFFFAOYSA-N
pH	8.0±0.2 (25 °C)
product line	BioUltra
Quality Level	200
SMILES string	NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
UV absorption	λ: 260 nm A_max: 0.04
	λ: 280 nm A_max: 0.01

Application:	TE buffer is useful for general extraction, suspension, washing, and storage buffer and in the preparation of DNA or RNA. Commonly used for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain reaction (PCR) applications.
Features and Benefits:	• BioUltra grade - suitable for many molecular biology applications • DNase, RNase, phosphatase, and protease-free • High purity and free of trace metals
General description:	Tris EDTA is a biological buffer in research. It is a combination of Tris base and EDTA, a metal chelator. Tris provides buffering capacity, maintaining a stable pH in biological samples, while EDTA helps prevent metal ion interference by chelating divalent metal ions. Tris EDTA is often used in molecular biology techniques, such as DNA and RNA sample preparation, electrophoresis, and enzymatic reactions. It helps maintain the stability and integrity of nucleic acids by inhibiting the activity of nucleases and metal-dependent enzymes.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41105319