Trizma^® hydrochloride buffer solution

SIAL/93313 - BioUltra, for molecular biology, pH 7.4

Synonym: Tris(hydroxymethyl)aminomethane hydrochloride buffer solution

MDL Number: MFCD00012590
Product Type: Chemical

λ	neat
cation traces	Al: ≤1 mg/kg
	As: ≤0.1 mg/kg
	Ba: ≤1 mg/kg
	Bi: ≤1 mg/kg
	Ca: ≤5 mg/kg
	Cd: ≤1 mg/kg
	Co: ≤1 mg/kg
	Cr: ≤1 mg/kg
	Cu: ≤1 mg/kg
	Fe: ≤1 mg/kg
	K: ≤20 mg/kg
	Li: ≤1 mg/kg
	Mg: ≤1 mg/kg
	Mn: ≤1 mg/kg
	Mo: ≤1 mg/kg
	Na: ≤20 mg/kg
	Ni: ≤1 mg/kg
	Pb: ≤1 mg/kg
	Sr: ≤1 mg/kg
	Zn: ≤1 mg/kg
concentration	1.0 M tris-HCl
density	1.05 g/mL at 20 °C
form	liquid
grade	for molecular biology
impurities	DNases, none detected
	insoluble matter, passes filter test
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
pH	7.4±0.1 (25 °C)
product line	BioUltra
Quality Level	100
SMILES string	Cl.NC(CO)(CO)CO
UV absorption	λ: 260 nm A_max: 0.03
	λ: 280 nm A_max: 0.04

Application:	Reduced glutathione inhibits rabbit and rat skeletal muscle lactate dehydrogenase and prevents dinitrophenol induced extracellular acidification by an epithelial cell line.: This study investigates how Trizma^® hydrochloride buffer solution maintains pH stability, critical for accurately assessing the biochemical interactions in muscle and epithelial cells (Wimberger et al., 1997 ).
Legal Information:	Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes:	Buffer component (SET buffer) in the preparation of RNA

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.05 g/mL at 20 °C
UNSPSC	12000000