TRIS Glycine buffer solution
SIAL/93321 - BioUltra, 10× concentrate
Synonym: Blotting buffer solution
Product Type: Chemical
| λ | neat |
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤500 mg/kg | |
| NH4+: ≤200 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| composition | glycine, 1.92 M |
| TRIS, 0.25 M | |
| form | liquid |
| ign. residue | ≤0.2% |
| impurities | insoluble matter, passes filter test |
| proteases, none detected | |
| pH | 8.5-8.7 |
| product line | BioUltra |
| quality | 10× concentrate |
| Quality Level | 100  |
| UV absorption | λ: 260 nm Amax: 0.1 |
| λ: 280 nm Amax: 0.1 |
| Application: | Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications. |
| Other Notes: | Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |