Trypan Blue solution

SIAL/93595 - 0.4%, suitable for microscopy

CAS Number: 72-57-1
Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight: 960.81
MDL Number: MFCD00003969
Linear Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-93595-50ML	50 mL	$64.40 1/EA		Add To Favorites
45-93595-250ML	250 mL	$131.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε_max	1.4 at 603 nm in methanol
application(s)	diagnostic assay manufacturing hematology histology
color	blue to very dark blue
concentration	0.4%
density	1.007 g/mL at 20 °C
form	liquid
InChI	1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key	GLNADSQYFUSGOU-UHFFFAOYSA-J
Quality Level	200
shelf life	limited shelf life, expiry date on the label
SMILES string	[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
storage temp.	room temp
suitability	suitable for microscopy
technique(s)	microbe id \| staining: suitable

Application:	Trypan blue applications include:• it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. • employed as a collagen stain in a Van Gieson procedure • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. • used as a fluorescent tracer of cell populations in embryology • used as a tumor promoter modulating permeability of lysosomal membranes Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.
Biochem/physiol Actions:	Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
General description:	Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.
Physical form:	0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Symbol	GHS08
Signal word	Danger
Hazard statements	H350
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	T
Risk Statements	45
Safety Statements	53-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.007 g/mL at 20 °C
Storage Temp.	room temp
UNSPSC	12171500

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