Trypan Blue solution

SIAL/93595 - 0.4%, for microscopy

CAS Number: 72-57-1
Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight: 960.81
MDL Number: MFCD00003969
Linear Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-93595-50ML	50 mL	$62.20 1/EA		Add To Favorites
45-93595-250ML	250 mL	$126.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε_max	1.4 at 603 nm in methanol
application(s)	diagnostic assay manufacturing hematology histology
concentration	0.4%
density	1.007 g/mL at 20 °C
form	liquid
grade	for microscopy
InChI	1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key	GLNADSQYFUSGOU-UHFFFAOYSA-J
Quality Level	200
shelf life	limited shelf life, expiry date on the label
SMILES string	[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
storage temp.	room temp

Application:	Trypan blue is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
Biochem/physiol Actions:	Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
General description:	Trypan Blue is a tetrasulfonated anionic, hydrophilic dye.
Physical form:	0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Symbol	GHS08
Signal word	Danger
Hazard statements	H350
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	T
Risk Statements	45
Safety Statements	53-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.007 g/mL at 20 °C
Storage Temp.	room temp
UNSPSC	12171500

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