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2-Mercaptoethanol

SIAL/97622 - for HPLC derivatization, LiChropur, ≥99.0% (GC)

Synonym: β-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol

CAS Number: 60-24-2
Empirical Formula (Hill Notation): C2H6OS
Molecular Weight: 78.13
EC Number: 200-464-6
MDL Number: MFCD00004890
Linear Formula: HSCH2CH2OH
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-97622-1ML 1 mL
$38.20
1/EA
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45-97622-10X1ML 1 mL
$151.00
10/EA
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45-97622-10ML 10 mL
$75.30
1/EA
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assay ≥99.0% (GC)
bp 157 °C (lit.)
concentration 14.3 M (pure liquid)
density 1.114 g/mL at 25 °C (lit.)
expl. lim. 18 %
form liquid
grade for HPLC derivatization
InChI 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
InChI key DGVVWUTYPXICAM-UHFFFAOYSA-N
quality LiChropur
Quality Level 100 
refractive index n20/D 1.500 (lit.)
  n20/D 1.500-1.502
SMILES string OCCS
storage temp. 2-8°C
technique(s) HPLC: suitable
vapor density 2.69 (vs air)
vapor pressure 1 mmHg ( 20 °C)
Application: BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Application: suitable for derivatisation of Amine groups
General description: 2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.
Legal Information: LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
Purity ≥99.0% (GC)
bp 157 °C (lit.)
Density 1.114 g/mL at 25 °C (lit.)
Refractive Index n20/D 1.500 (lit.); n20/D 1.500-1.502
Storage Temp. 2-8°C
UNSPSC 12000000

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