Agarose, low gelling temperature
SIAL/A0701 - Type VII-A
Synonym: 2-Hydroxyethyl agarose
CAS Number: 39346-81-1
MDL Number: MFCD00081294
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A0701-100G
This image depicts SKU: A0701-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A0701-25G
This image depicts SKU: A0701-25G
| anion traces | sulfate (SO42-): ≤0.4% |
| ash | ≤0.4% |
| EEO | ≤0.12 |
| form | powder |
| gel strength | ≥250 g/cm2 (1% gel) |
| impurities | ≤7% water |
| mp | 65 °C±1 °C |
| Quality Level | 200  |
| technique(s) | electrophoresis: suitable |
| transition temp | gel point 26 °C ±2 °C (1.5% gel) |
| turbidity | ≤4 NTU (1.5% gel) |
| type | Type VII-A |
| Analysis Note: | The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection. |
| Application: | Excellent for in-gel enzymatic reactions and cloning assays and for recovery of heat-labile samples after electrophoresis Agarose has been used: • to encapsulate Escherichia coli on a hydrogel in tissue culture • to entrap Aliivibrio fischeri on a disposable card involved in designing of toxicity biosensors • as a the dispersed phase of emulsion during preparation agar beads |
| Biochem/physiol Actions: | Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic) It is also employed to determine cross reaction in immunoelectrophoresis (IEP) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied. Agarose is used to make gel plates or overlays for cells in tissue culture. In addition, it is also used to form a gel matrix (either beaded and/or crosslinked) which can be used in chromatographic separations. |
| General description: | Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers |
| Packaging: | 25, 100 g in poly bottle |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 65 °C±1 °C |
| UNSPSC | 41105317 |