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Agarose

SIAL/A2576 - Ultra-low Gelling Temperature, molecular biology grade

Synonym: 3,6-Anhydro-α-L-galacto-β-D-galactan

CAS Number: 9012-36-6
EC Number: 232-731-8
MDL Number: MFCD00081294
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A2576-5G 5 g
$106.00
1/EA
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45-A2576-25G 25 g
$357.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: A2576-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: A2576-5G

 

anion traces sulfate (SO42-): ≤0.14%
ash ≤0.4%
EEO ≤0.12
form powder
gel strength ≥300 g/cm2 (1.5% gel)
grade Molecular Biology
impurities ≤7% water
InChI 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key MJQHZNBUODTQTK-WKGBVCLCSA-N
mp ≤62 °C
Quality Level 200 
technique(s) electrophoresis: suitable
transition temp gel point ≤20 °C (1.5% gel)
type Type IX-A
Analysis Note: The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application: Agarose has been used:
• in thaumatin and elastase protein crystallization
• in microcasting using a polydimethylsiloxane (PDMS) mold with micro-channel
• in agarose micro-confinement array

Application: Agarose is super low melting point and recommended for use in capillary electrophoresis, tissues & cell culture, and viral plaque assay.
Application: Yields unusually strong gels for an ultra-low gelling agarose. Ideal for electrophoresis of heat-labile samples and for growth of hybridomas or other cell lines.
General description: Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.
Packaging: 5, 25 g in poly bottle
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp ≤62 °C
UNSPSC 41105317

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