Agarose
SIAL/A4718 - for molecular biology
Synonym: 3,6-Anhydro-α-L-galacto-β-D-galactan
CAS Number: 9012-36-6
EC Number: 232-731-8
MDL Number: MFCD00081294
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A4718-100G
This image depicts SKU: A4718-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A4718-25G
This image depicts SKU: A4718-25G
| biological source | algae (marine) |
| EEO | ≤0.05 |
| foreign activity | DNase, RNase, NICKase, none detected |
| form | powder |
| grade | for molecular biology |
| InChI | 1S/C24H38O19/c25-1-5-9(27 |
| InChI key | MJQHZNBUODTQTK-WKGBVCLCSA |
| mp | ≤75 °C (3% gel) |
| Quality Level | 200  |
| storage temp. | room temp |
| technique(s) | electrophoresis: suitable |
| transition temp | gel point ≤35 °C (3% gel) |
| Analysis Note: | The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection. |
| Application: | Agarose has been used in the preparation of microgel slides employed in lysis and electrophoresis. It has also been used in the gel electrophoresis to separate restriction enzyme digested products in catechol-O-methyltransfer |
| Application: | Ideal for the separation of small DNA fragments (e.g., PCR products) differing in size by as little as 2% and compares to the resolution of DNA in polyacrylamide gels. High resolution agarose is also ideal for resolving amplification fragment length polymorphisms (AmpFLP), short tandem repeats (STR) and tri- and tetranucleotide repeats. Low ethidium bromide and SYBR Green background staining |
| General description: | Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically. |
| Packaging: | 25, 100 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | ≤75 °C (3% gel) |
| Storage Temp. | room temp |
| UNSPSC | 41105317 |