Agarose

SIAL/A6877 - Medium EEO, for molecular biology

Synonym: 3,6-Anhydro-α-L-galacto-β-D-galactan; Agarose ME

CAS Number: 9012-36-6
EC Number: 232-731-8
MDL Number: MFCD00081294
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A6877-25G	25 g	$120.00 1/EA	Add To Favorites
45-A6877-50G	50 g	$200.00 1/EA	Add To Favorites
45-A6877-100G	100 g	$361.00 1/EA	Add To Favorites
45-A6877-250G	250 g	$695.00 1/EA	Add To Favorites
45-A6877-500G	500 g	$1082.39 1/EA	Add To Favorites
45-A6877-1KG	1 kg	$2110.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: A6877-100G

Properties
Descriptions
Safety Info.
Basic Data

biological source	algae (marine)
EEO	0.16-0.19
form	powder
gel strength	≥1000 g/cm² (1.0% gel)
grade	for molecular biology
impurities	≤10% moisture content
InChI	1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key	MJQHZNBUODTQTK-WKGBVCLCSA-N
Quality Level	200
transition temp	congealing temperature 34.5-37.5 °C (1.5% gel)
type	Type II

Analysis Note:	The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application:	Agarose has been used: • in gel electrophoresis • in the preparation of 2x agarose solution used along with 2x supplemented-MEM media as an overlay medium in plaque assay • in an immunodiffusion test for Paracoccidioidomycosis
General description:	Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible, possesses variable mechanical and diffusion properties.
Packaging:	1 kg in poly bottle
Packaging:	5, 10, 25, 50, 100, 250, 500 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41105317

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