Agarose

SIAL/A7174 - High Gelling Temperature

Synonym: 3,6-Anhydro-α-L-galacto-β-D-galactan

CAS Number: 9012-36-6
EC Number: 232-731-8
MDL Number: MFCD00081294
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A7174-10G	10 g	$180.00 1/EA	Add To Favorites
45-A7174-25G	25 g	$356.00 1/EA	Add To Favorites

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This image depicts SKU: A7174-25G

Properties
Descriptions
Safety Info.
Basic Data

anion traces	sulfate (SO₄^2-): ≤0.20%
ash	≤0.60%
EEO	≤0.14
form	powder
gel strength	≥900 g/cm² (1% gel)
impurities	≤10% water
InChI	1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key	MJQHZNBUODTQTK-WKGBVCLCSA-N
Quality Level	200
transition temp	gel point 41 °C ±1.5 °C (1.5% gel)
type	Type VI-A

Analysis Note:	The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application:	Quality and applicability of cadaveric donor eyes for molecular biology research: An Indian experience.: This study discusses the use of agarose in preparing donor eyes for molecular biology research, highlighting its effectiveness in preserving samples for high-quality genetic analysis (Kaur et al., 2024 ). Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block.: Agarose is used to enhance the immunocytochemistry process, providing a more efficient and clear diagnostic method for hematolymphoid diseases (Choi et al., 2024 ). Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.: Demonstrates the use of low-melting point agarose as a versatile medium for advanced imaging and proteomic analysis, contributing significantly to analytical biochemistry (McDonnell et al., 2023 ). Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex.: Agarose is utilized in the study of cellular mechanisms for cancer research, underscoring its importance in experimental setups requiring precise biological simulations (Guzhova et al., 2023 ).
Packaging:	10, 25 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41105317

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