Acridine Orange hydrochloride solution
SIAL/A8097 - 10 mg/mL in H2O, >95.0% (HPLC)
Synonym: 3,6-
CAS Number: 65-61-2
Empirical Formula (Hill Notation): C17H19N3 · HCl
Molecular Weight: 301.81
MDL Number: MFCD00012660
Linear Formula: C17H19N3 · HCl
Product Type: Chemical
| εmax | 47-54 at 486-492 nm in water |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | >95.0% (HPLC) |
| color | orange to red |
| concentration | 10 mg/mL in H2O |
| form | solution |
| InChI | 1S/C17H19N3.ClH/c1-19(2)1 |
| InChI key | VSTHNGLPHBTRMB-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | Cl[H].CN(C)c1ccc2cc3ccc(c |
| storage temp. | room temp |
| Application: | Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes. |
| Biochem/physiol Actions: | Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95.0% (HPLC) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |