Bovine Serum Albumin
SIAL/A8654 - dimer mol wt ~132 kDa
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
| assay | 0.9-1.4 mg per vial protein basis (biuret) |
| biological source | bovine |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| dimer ~132 kDa | |
| monomer ~66 kDa | |
| origin | USA origin |
| pH | 5.2 |
| purified by | cold ethanol fractionation |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Marker for non-denaturing PAGE |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Reconstitution: | 0.9-1.4 mg/mL after reconstitution with 1 mL of water |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 0.9-1.4 mg per vial protein basis (biuret) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |