Bovine Serum Albumin
SIAL/A9056 - heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9056-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9056-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9056-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9056-500G
| assay | ≥96% (agarose gel electrophoresis) |
| biological source | bovine |
| foreign activity | BT virus, none detected |
| growth of Borrelia burgdorferi in BSK II medium (Suitable for) | |
| Microbiological test (pass) | |
| VSV virus, none detected | |
| form | lyophilized powder |
| impurities | ≤5.0% Loss on drying |
| loss | ≤5% |
| mol wt | ~66 kDa |
| origin | USA origin |
| packaging | poly bottle of |
| pH | 7 |
| purified by | heat shock fractionation |
| Quality Level | 200  |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02769  |
| Application: | Bovine Serum Albumin has been used as a supplement in Dulbecco′s modified eagle medium (DMEM). It has also been used in immunostaining of cell arrays. |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| General description: | Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease. |
| Packaging: | 10, 50, 100, 500 g in poly bottle |
| Preparation Note: | Prepared using heat shock fractionation |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| Suitability: | Suitable for growth of Borrelia burgdorferi in BSK II medium |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96% (agarose gel electrophoresis) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |