Bovine Serum Albumin
SIAL/A9085 - lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9085-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9085-5G
| assay | ≥96% (agarose gel electrophoresis) |
| biological source | bovine |
| foreign activity | Blue tongue virus, none detected |
| Vesicular Statitis virus, none detected | |
| form | lyophilized powder |
| impurities | IgG free |
| ≤25 ng/mg protein IgG | |
| mol wt | ~66 kDa |
| origin | USA origin |
| packaging | poly bottle of |
| pH | 7 |
| purified by | heat shock fractionation |
| Quality Level | 200  |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunofluorescence: suitable | |
| UniProt accession no. | P02769  |
| Application: | Bovine Serum Albumin has been used in: • the washing of the rabbit tissue sections in immunofluorescence microscopy • incubation of red blood cells (RBC) for flow cytometry analysis • as a component of Hanks′ balanced salt solution with Ca2+ and Mg2+ (HBSS++) for eosin-5-maleimide staining and RBC washing in magnetic circular dichroism measurements |
| Biochem/physiol Actions: | Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is used as a blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA shares structural features with human serum albumin. Its high solubility, low cost and purity and interaction with surfactant is exploited in cosmetic and pharmaceutical industry. |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Features and Benefits: | • Essentially IgG free (immunoglobulin-free) • Heat shock fractionated |
| General description: | Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. BSA belongs to the serum albumins family. It has 17-disulfide bonds. |
| Packaging: | 5, 25 g in poly bottle |
| Preparation Note: | Prepared using heat shock fractionation. |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96% (agarose gel electrophoresis) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |