Bromophenol Blue
SIAL/B0126
Synonym: 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein; Bromophenol Blue (BPB); Bromphenol Blue Sultone Form
CAS Number: 115-39-9
Empirical Formula (Hill Notation): C19H10Br4O5S
Molecular Weight: 669.96
EC Number: 204-086-2
MDL Number: MFCD00005875
Linear Formula: C19H10Br4O5S
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
form | powder |
InChI | 1S/C19H10Br4O5S/c20-12-5- |
InChI key | UDSAIICHUKSCKT-UHFFFAOYSA |
mp | 273 °C |
Quality Level | 200 ![]() |
SMILES string | Oc1c(Br)cc(cc1Br)C2(OS(=O |
solubility | methanol: 10 mg/mL |
storage temp. | room temp |
technique(s) | titration: suitable |
visual transition interval | 3.0-4.6, yellow-green to blue |
Application: | Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier. |
General description: | Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonpht |
Packaging: | 25 g in glass bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 273 °C |
Storage Temp. | room temp |
UNSPSC | 12171500 |