bisBenzimide H 33342 trihydrochloride
SIAL/B2261 - ≥98% (HPLC and TLC)
Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride; HOE 33342; Hoechst 33342; bisBenzimide
CAS Number: 875756-97-1
Empirical Formula (Hill Notation): C27H28N6O · 3HCl · xH2O
Molecular Weight: 561.93 (anhydrous basis)
MDL Number: MFCD00012678
Linear Formula: C27H28N6O · 3HCl · xH2O
Product Type: Chemical
Immunofluorescence 293 cells transfected with HA tagged fusion protein were fixed, permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100 and stained with 1 μg/mL bisBenzimide H 33342 trihydrochloride (Cat. No. B2261) (blue) to stain nuclei. 40× magnification.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2261-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2261-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2261-500MG
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥98% (HPLC and TLC) |
| form | powder |
| InChI | 1S/C29H32N6O.3ClH/c1-3-36 |
| InChI key | FYEVKHPLBHLWHK-UHFFFAOYSA |
| pH | 1.7 (20 °C) |
| Quality Level | 300  |
| SMILES string | Cl[H].Cl[H].Cl[H].CCOc1cc |
| solubility | H2O: 20 mg/mL |
| phosphate buffer: precipitates | |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| Application: | Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in living cells. It has been used to detect BrdU incorporation into cells and in studying the initial stages apoptosis and cellcycle distribution., Chromosomes that are dividing or replicating will not stain with this dye. |
| Application: | Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Excitation max. = 346 nm Emission max. = 460 nm |
| Biochem/physiol Actions: | Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA. |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 500 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Physical properties: | Fluorescent properties of bisBenzimide H 33342: Free dye: Excitation maximum = 340 nm, Emission maximum = 510 nm (5 mM HEPES, 10 mM NaCl, pH 7.0) DNA complex: Excitation maximum = 355 nm, Emission maximum = 465 nm (5 mM HEPES, 10 mM NaCl, pH 7.0) |
| Preparation Note: | This product is soluble in water (50 mg/ml), yielding a clear solution. The pH of a 2% solution is 1.9. It has been observed that this material will precipitate from phosphate buffer solutions. Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C. |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38 |
| Safety Statements | 22-37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC and TLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |