bisBenzimide H 33258
SIAL/B2883 - ≥98% (HPLC and TLC)
Synonym: 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride; 2-
CAS Number: 23491-45-4
Empirical Formula (Hill Notation): C25H24N6O · 3HCl
Molecular Weight: 533.88
EC Number: 245-690-6
MDL Number: MFCD00012679
Linear Formula: C25H24N6O · 3HCl
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1 μg/mL bisBenzimide H 33258 (Cat. No. B2883) to stain nuclei.
Immunofluorescence : SCK-CEN Mouse cortex primary neurons cultured for 10 days were stained for PSD95, Β-Tubulin III and nuclei. For PSD95 Monoclonal Anti-PSD95 antibody produced in Mouse (Cat. No. P246) and Anti-Mouse IgG (whole molecule)–FITC antibody produced in Goat (Cat. No. F2012) (green) were used. For Β-Tubulin III Anti-Β-Tubulin III antibody produced in Rabbit (Cat. No. T2200) and Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in Goat (Cat. No. T6778) (red). Nuclei were stained with Hoechst B2883 (blue/purple). Submitted by Dr G. Pani, Studiecentrum voor Kernenergie-Centre d′Etude d′Energie Nucleaire
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2883-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2883-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2883-500MG
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥98% (HPLC and TLC) |
| form | powder |
| InChI | 1S/C27H28N6O.3ClH.H2O/c1- |
| InChI key | OWRSPPSBNWJJAR-UHFFFAOYSA |
| mp | 280 °C |
| Quality Level | 200  |
| SMILES string | Cl[H].Cl[H].Cl[H].[H]O[H] |
| solubility | H2O: 10 mg/mL |
| phosphate buffer: precipitates | |
| water and ethanol: 10 mg/mL | |
| storage temp. | −20°C |
| suitability | passes application test for fluorescence |
| Application: | bisBenzimide H 33258 has been used for nuclear staining in cells. |
| Application: | bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry. Excitation max. = 346 nm Emission max. = 460 nm |
| Biochem/physiol Actions: | bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA. |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 500 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC and TLC) |
| mp | 280 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |