Bile salts mixture
SIAL/B3426 - suitable for microbiology
Synonym: Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture
Product Type: Chemical
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| application(s) | microbiology |
| color | yellow to brownish-yellow |
| form | powder |
| loss | ≤6% loss on drying |
| packaging | pkg of 100 g |
| pH | 7±0.5 |
| Quality Level | 100  |
| solubility | distilled water: freely soluble |
| sterility | non-sterile |
| storage temp. | 10-30°C |
| Application: | Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species. |
| General description: | Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae. |
| Preparation Note: | Extracted from purified, fresh bile. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 10-30°C |
| UNSPSC | 41106212 |