Vitamin B12 assay medium
SIAL/B3801 - NutriSelect® Plus, suitable for microbiology
Synonym: Vitamin B12 Assay Medium, Cyanocobalamin Assay Medium
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B3801-100G
| application(s) | agriculture food and beverages pharmaceutical |
| microbiology | |
| color | light amber |
| final pH | 6.1±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| packaging | bottle of 100 g |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| suitability | nonselective for Lactobacillus spp. |
| technique(s) | microbiological culture: suitable |
| Application: | Vitamin B12 Assay Medium is a Vitamin B12 free medium recommended for microbiological assay of Vitamin B12 from various test samples |
| Components: | Ingredients (g/L) Casein acid hydrolysate, 15.00 Dextrose, 40.00 Asparagine, 0.20 Sodium acetate, 20.00 Ascorbic acid, 4.00 Adenine sulfate, 0.02 Guanine hydrochloride, 0.02 Uracil, 0.02 Xanthine, 0.02 Riboflavin (Vitamin B2), 0.001 Thiamine hydrochloride, 0.001 Biotin, 0.00001 Niacin, 0.002 p-Amino benzoic acid, 0.002 Calcium pantothenate, 0.001 Pyridoxine hydrochloride, 0.004 Pyridoxal hydrochloride, 0.004 Pyridoxamine hydrochloride, 0.0008 Folic acid, 0.0002 Monopotassium phosphate, 1.00 Dipotassium phosphate, 1.00 Magnesium sulfate, 0.40 Sodium chloride, 0.02 Ferrous sulfate, 0.02 Manganese sulfate, 0.02 Polysorbate 80, 2.00 |
| Features and Benefits: | NutriSelect® Plus are powdered culture media offering• Quality control with growth promotion test according to industry standards • Quality assurance acc. to ISO 9001 • Cost-efficient powdered media |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Vitamin B12 Assay Medium is a Vitamin B12 free medium containing all other vitamins and nutrients essential for the growth of Lactobacillus leichmannii ATCC 7830 as the test organism. Cyanocobalamin (Vitamin B12, Cat.No. 95190 ) is added in increasing concentrations giving a growth response that can be measured turbidimetrically or acidimetrically. A standard curve is plotted with absorbance as a function of the vitamin B12 concentration. The concentration of vitamin B12 in the test sample is calculated based on the interpretation of the standard curve. Lactobacillus species grow poorly on non-selective culture media and require special nutrients for their growth. Vitamin assay media are prepared for use in the microbiological assay of vitamins. Three types of media used for the microbiological assay of vitamins are: • The maintenance media used for carrying the stock culture • The inoculum media for preparation of the inoculum • The assay media for quantitation of the vitamin under test. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 8.5 g of Vitamin B12 Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 43107) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41171608 |