Vitamin B12 assay medium
SIAL/B3801 - suitable for microbiology, NutriSelect® Plus
Product Type: Chemical
application(s) | agriculture food and beverages pharmaceutical |
microbiology | |
final pH | 6.1±0.2 (25 °C) |
form | powder |
manufacturer/tradename | NutriSelect® Plus |
Quality Level | 200 |
shelf life | limited shelf life, expiry date on the label |
sterility | non-sterile |
storage temp. | 2-8°C |
suitability | nonselective for Lactobacillus spp. |
technique(s) | microbiological culture: suitable |
Application: | Recommended for microbiological assay of Vitamin B12. |
Components: | Ingredients (g/L) Casein acid hydrolysate, 15.00 Dextrose, 40.00 Asparagine, 0.20 Sodium acetate, 20.00 Ascorbic acid, 4.00 Adenine sulfate, 0.02 Guanine hydrochloride, 0.02 Uracil, 0.02 Xanthine, 0.02 Riboflavin (Vitamin B2), 0.001 Thiamine hydrochloride, 0.001 Biotin, 0.00001 Niacin, 0.002 p-Amino benzoic acid, 0.002 Calcium pantothenate, 0.001 Pyridoxine hydrochloride, 0.004 Pyridoxal hydrochloride, 0.004 Pyridoxamine hydrochloride, 0.0008 Folic acid, 0.0002 Monopotassium phosphate, 1.00 Dipotassium phosphate, 1.00 Magnesium sulfate, 0.40 Sodium chloride, 0.02 Ferrous sulfate, 0.02 Manganese sulfate, 0.02 Polysorbate 80, 2.00 |
Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
Preparation Note: | Suspend 8.5 g of Vitamin B12 Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 43107) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the medium. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 41171608 |