Bromobimane
SIAL/B4380 - ≥97% (HPLC)
Synonym: Monobromobimane
CAS Number: 71418-44-5
Empirical Formula (Hill Notation): C10H11BrN2O2
Molecular Weight: 271.11
MDL Number: MFCD00036951
Linear Formula: C10H11BrN2O2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B4380-100MG
| ε (extinction coefficient) | 4.6-5.1 at 396-398 nm in H2O |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥97% (HPLC) |
| color | yellow |
| form | powder |
| InChI | 1S/C10H11BrN2O2/c1-5-7(3) |
| InChI key | AHEWZZJEDQVLOP-UHFFFAOYSA |
| mp | 161 °C |
| Quality Level | 200  |
| SMILES string | CC1=C(C)C(=O)N2N1C(CBr)=C |
| solubility | acetonitrile: 20 mg/mL |
| storage temp. | −20°C |
| Application: | Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-s Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro. It has been used for the labeling of proteins containing thiol groups. |
| Biochem/physiol Actions: | Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection. |
| General description: | Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction. |
| Packaging: | 25, 100 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (HPLC) |
| mp | 161 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |