Bromobimane

SIAL/B4380 - ≥97%

Synonym: Monobromobimane

CAS Number: 71418-44-5
Empirical Formula (Hill Notation): C₁₀H₁₁BrN₂O₂
Molecular Weight: 271.11
MDL Number: MFCD00036951
Linear Formula: C₁₀H₁₁BrN₂O₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B4380-25MG	25 mg	$170.00 1/EA	Add To Favorites
45-B4380-100MG	100 mg	$440.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B4380-100MG

Properties
Descriptions
Safety Info.
Basic Data

ε (extinction coefficient)	4.6-5.1 at 396-398 nm in H₂O
application(s)	diagnostic assay manufacturing hematology histology
assay	≥97%
color	yellow
form	powder
InChI	1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
InChI key	AHEWZZJEDQVLOP-UHFFFAOYSA-N
mp	161 °C
Quality Level	200
SMILES string	CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
solubility	acetonitrile: 20 mg/mL
storage temp.	−20°C

Application:	Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria. Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro. It has been used for the labeling of proteins containing thiol groups.
Biochem/physiol Actions:	Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.
General description:	Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.
Packaging:	25, 100 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥97%
mp	161 °C
Storage Temp.	−20°C
UNSPSC	12171500

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