Bromobimane
SIAL/B4380 - ≥97%
Synonym: Monobromobimane
CAS Number: 71418-44-5
Empirical Formula (Hill Notation): C10H11BrN2O2
Molecular Weight: 271.11
MDL Number: MFCD00036951
Linear Formula: C10H11BrN2O2
Product Type: Chemical
ε (extinction coefficient) | 4.6-5.1 at 396-398 nm in H2O |
application(s) | diagnostic assay manufacturing hematology histology |
assay | ≥97% |
form | powder |
InChI | 1S/C10H11BrN2O2/c1-5-7(3) |
InChI key | AHEWZZJEDQVLOP-UHFFFAOYSA |
mp | 161 °C |
Quality Level | 200 ![]() |
SMILES string | CC1=C(C)C(=O)N2N1C(CBr)=C |
solubility | acetonitrile: 20 mg/mL |
storage temp. | −20°C |
Application: | Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-s Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro. It has been used for the labeling of proteins containing thiol groups. |
Biochem/physiol Actions: | Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection. |
General description: | Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction. |
Packaging: | 25, 100 mg in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥97% |
mp | 161 °C |
Storage Temp. | −20°C |
UNSPSC | 12171500 |