Biotin

SIAL/B4501 - ≥99% (HPLC), lyophilized powder

Synonym: D-Biotin; Bios II; Coenzyme R; Vitamin B7; Vitamin H

CAS Number: 58-85-5
Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₃S
Molecular Weight: 244.31
EC Number: 200-399-3
MDL Number: MFCD00005541
Linear Formula: C₁₀H₁₆N₂O₃S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B4501-100MG	100 mg	$30.10 1/EA	Add To Favorites
45-B4501-500MG	500 mg	$71.20 1/EA	Add To Favorites
45-B4501-1G	1 g	$101.00 1/EA	Add To Favorites
45-B4501-5G	5 g	$465.00 1/EA	Add To Favorites
45-B4501-10G	10 g	$797.00 1/EA	Add To Favorites
45-B4501-25G	25 g	$1700.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: B4501-25G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (HPLC)
biological source	synthetic (organic)
color	white to off-white
form	lyophilized powder
InChI	1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
InChI key	YBJHBAHKTGYVGT-ZKWXMUAHSA-N
mp	231-233 °C (lit.)
Quality Level	300
SMILES string	[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
solubility	ammonium hydroxide: 50 mg/mL (2 M)
storage temp.	2-8°C
technique(s)	HPLC: suitable

Application:	Biotin may be used to elute proteins from avidin/streptavidin resins. Biotin has been used:• for culturing of oligodendrocytes. • as a vitamin supplement for the growth of Bacillus species. • for blocking endogenous biotin during immunohistology procedures.
Biochem/physiol Actions:	Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are is required for various applications. Biotin also works as a cofactor/coenzyme of carboxylating enzymes, such as pyruvate carboxylase, acetyl-CoA carboxylases 1 and 2, 3-methylcrotonyl-CoA carboxylase (MCC), and propionyl-CoA carboxylase (PCC). It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A, etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry, and flow cytometry. This approach is also applied for to the purification and characterization of various molecules of interest. Biotin is involved in the epigenetic regulation of genes, cell signaling, and the structure of chromatin.
General description:	Biotin is a water-soluble vitamin. Mammals depend on biotin via plant and microbial dietary sources. Biotin consists of two rings that are attached to valeric acid moiety as a side chain. One of the rings is attached to ureido group and the other ring is the tetrahydrothiophene group.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1, 5, 10, 25 g in poly bottle
Packaging:	100, 500 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (HPLC)
mp	231-233 °C (lit.)
Storage Temp.	2-8°C
UNSPSC	12352201

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