Biotin
SIAL/B4501 - ≥99% (HPLC), lyophilized powder
Synonym: D-Biotin; Bios II; Coenzyme R; Vitamin B7; Vitamin H
CAS Number: 58-85-5
Empirical Formula (Hill Notation): C10H16N2O3S
Molecular Weight: 244.31
EC Number: 200-399-3
MDL Number: MFCD00005541
Linear Formula: C10H16N2O3S
Product Type: Chemical
assay | ≥99% (HPLC) |
biological source | synthetic (organic) |
color | white to off-white |
form | lyophilized powder |
InChI | 1S/C10H16N2O3S/c13-8(14)4 |
InChI key | YBJHBAHKTGYVGT-ZKWXMUAHSA |
mp | 231-233 °C (lit.) |
Quality Level | 300 |
SMILES string | [H][C@]12CS[C@@H](CCCCC(O |
solubility | ammonium hydroxide: 50 mg/mL (2 M) |
storage temp. | 2-8°C |
technique(s) | HPLC: suitable |
Application: | Biotin may be used to elute proteins from avidin/streptavidin resins. • It has been used for culturing of oligodendrocytes. • It has been used as a vitamin supplement for the growth of Bacillus species. • It has been used for blocking endogenous biotin during immunohistology procedures. |
Biochem/physiol Actions: | Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest. |
Packaging: | 1, 5, 10, 25 g in poly bottle |
Packaging: | 100, 500 mg in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% (HPLC) |
mp | 231-233 °C (lit.) |
Storage Temp. | 2-8°C |
UNSPSC | 12352201 |