Biotin-4-Fluorescein

SIAL/B9431

Empirical Formula (Hill Notation): C₃₃H₃₂N₄O₈S
Molecular Weight: 644.69
Linear Formula: C₃₃H₃₂N₄O₈S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-B9431-5MG	5 mg	$536.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε (extinction coefficient)	≥70 at 491-497 nm in phosphate buffer at 0.05 M
application(s)	diagnostic assay manufacturing hematology histology
assay	≥95.0% (HPLC)
form	powder
mol wt	644.71
Quality Level	100
SMILES string	OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56
storage temp.	room temp

Application:	Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.
Application:	Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.
Biochem/physiol Actions:	Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥95.0% (HPLC)
Storage Temp.	room temp
UNSPSC	12171500

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