Biotin-4-Fluorescein
SIAL/B9431 - ≥95.0% (HPLC)
Synonym: 5-
Empirical Formula (Hill Notation): C33H32N4O8S
Molecular Weight: 644.69
Linear Formula: C33H32N4O8S
Product Type: Chemical
| ε (extinction coefficient) | ≥70 at 491-497 nm in phosphate buffer at 0.05 M |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥95.0% (HPLC) |
| color | light orange to very dark orange |
| light yellow to very dark yellow | |
| form | powder |
| mol wt | 644.71 |
| Quality Level | 100  |
| SMILES string | OC(=O)c1cc(ccc1C2=C3C=CC( |
| storage temp. | room temp |
| Application: | Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin. |
| Application: | Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin. |
| Biochem/physiol Actions: | Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95.0% (HPLC) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |