Biotin-4-Fluorescein

SIAL/B9431 - ≥95.0% (HPLC)

Synonym: 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein; B4F; Biotin (5-fluorescein) conjugate

Empirical Formula (Hill Notation): C₃₃H₃₂N₄O₈S
Molecular Weight: 644.69
MDL Number: MFCD09264223
Linear Formula: C₃₃H₃₂N₄O₈S
Product Type: Chemical

ε (extinction coefficient)	≥70 at 491-497 nm in phosphate buffer at 0.05 M
application(s)	diagnostic assay manufacturing hematology histology
assay	≥95.0% (HPLC)
color	light orange to very dark orange
	light yellow to very dark yellow
form	powder
InChI	1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30
InChI key	UROXVNHIYQXTRA-LFERIPGTSA-N
mol wt	644.71
Quality Level	100
	200
SMILES string	OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56
storage temp.	room temp
technique(s)	titration: suitable

Application:	Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.
Application:	Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.
Biochem/physiol Actions:	Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable