Calcein AM solution
SIAL/C1359 - 4 mM in DMSO, ≥90% (HPLC), solution
CAS Number: 148504-34-1
Empirical Formula (Hill Notation): C46H46N2O23
Molecular Weight: 994.86
EC Number: 200-664-3
MDL Number: MFCD05861516
Linear Formula: C46H46N2O23
Product Type: Chemical
| λmax | 491-497 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥90% (HPLC) |
| concentration | 4 mM in DMSO |
| form | solution |
| InChI | 1S/C46H46N2O23/c1-25(49)6 |
| InChI key | XKFSBWQWNMZWFA-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | CC(=O)OCOC(=O)CN(CC(=O)OC |
| storage temp. | −20°C |
| Application: | In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells. |
| Biochem/physiol Actions: | Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery. |
| Biochem/physiol Actions: | Fluorescent cell permeable derivative of calcein. |
| Packaging: | 100 μL in glass bottle |
| Physical form: | Solution in 0.4% Calcein AM and 99.6% DMSO. |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 185.0 °F - closed cup |
| Flash Point(C) | 85 °C - closed cup |
| Purity | ≥90% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |