Calcein AM solution

SIAL/C1359 - 4 mM in DMSO, ≥90% (HPLC), solution

CAS Number: 148504-34-1
Empirical Formula (Hill Notation): C₄₆H₄₆N₂O₂₃
Molecular Weight: 994.86
EC Number: 200-664-3
MDL Number: MFCD05861516
Linear Formula: C₄₆H₄₆N₂O₂₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-C1359-100UL	100 µL	$327.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ_max	491-497 nm
application(s)	diagnostic assay manufacturing hematology histology
assay	≥90% (HPLC)
color	colorless
	white to off-white
concentration	4 mM in DMSO
form	solution
InChI	1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
InChI key	XKFSBWQWNMZWFA-UHFFFAOYSA-N
Quality Level	100
SMILES string	CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
storage temp.	−20°C

Application:	In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.
Biochem/physiol Actions:	Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.
Biochem/physiol Actions:	Fluorescent cell permeable derivative of calcein.
Packaging:	100 μL in glass bottle
Physical form:	Solution in 0.4% Calcein AM and 99.6% DMSO.

RIDADR	NA 1993 / PGIII
WGK Germany	WGK 2
Flash Point(F)	185.0 °F - closed cup
Flash Point(C)	85 °C - closed cup

Purity	≥90% (HPLC)
Storage Temp.	−20°C
UNSPSC	12171500

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