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7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

SIAL/C1484 - ≥95% (HPLC), solid

Synonym: 1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-; CPM

CAS Number: 76877-33-3
Empirical Formula (Hill Notation): C24H22N2O4
Molecular Weight: 402.44
MDL Number: MFCD00077334
Linear Formula: C24H22N2O4
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-C1484-25MG 25 mg
 $417.00
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application(s) diagnostic assay manufacturing
hematology
histology
assay ≥95% (HPLC)
color yellow
form solid
InChI 1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
InChI key YGIABALXNBVHBX-UHFFFAOYSA-N
Quality Level 100 
SMILES string CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O
solubility DMSO: soluble
  methanol: soluble
storage temp. −20°C
Application: 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:
• as a nontoxic substitute for conjugation with antibody(93)
• to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)
• in thermostability shift assay of recombinant protein(95)
Biochem/physiol Actions: Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.
General description: 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95% (HPLC)
Storage Temp. −20°C
UNSPSC 12171500

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