5-Carboxytetramethylrhodamine

SIAL/C2734

Synonym: 5-TAMRA

CAS Number: 91809-66-4
Empirical Formula (Hill Notation): C₂₅H₂₂N₂O₅
Molecular Weight: 430.45
MDL Number: MFCD01073638
Linear Formula: C₂₅H₂₂N₂O₅
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-C2734-10MG	10 mg	$598.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε (extinction coefficient)	≥85000-97000 at 539-545 nm in methanol
application(s)	diagnostic assay manufacturing hematology histology
assay	≥95% (HPLC)
fluorescence	λ_ex ~543 nm; λ_em ~570 nm in methanol
form	solid
InChI	1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
InChI key	YMZMTOFQCVHHFB-UHFFFAOYSA-N
Quality Level	100
SMILES string	N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C
solubility	water: slightly soluble
storage temp.	room temp

Application:	5-Carboxytetramethylrhodamine has been used in fluorophore characterization.
Biochem/physiol Actions:	5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.
General description:	An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥95% (HPLC)
Storage Temp.	room temp
UNSPSC	12171500

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