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5-Carboxytetramethylrhodamine

SIAL/C2734

Synonym: 5-TAMRA

CAS Number: 91809-66-4
Empirical Formula (Hill Notation): C25H22N2O5
Molecular Weight: 430.45
MDL Number: MFCD01073638
Linear Formula: C25H22N2O5
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-C2734-10MG 10 mg
$554.00
1/EA
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ε (extinction coefficient) ≥85000-97000 at 539-545 nm in methanol
application(s) diagnostic assay manufacturing
hematology
histology
assay ≥95% (HPLC)
fluorescence λex ~543 nm; λem ~570 nm in methanol
form solid
InChI 1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
InChI key YMZMTOFQCVHHFB-UHFFFAOYSA-N
Quality Level 100 
solubility water: slightly soluble
storage temp. room temp
Application: 5-Carboxytetramethylrhodamine has been used in fluorophore characterization.
Biochem/physiol Actions: 5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.
General description: An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95% (HPLC)
Storage Temp. room temp
UNSPSC 12171500

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