CHAPS hydrate

SIAL/C3023 - ≥98% (HPLC)

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate; CHAPS

CAS Number: 331717-45-4
Empirical Formula (Hill Notation): C₃₂H₅₈N₂O₇S · xH₂O
Molecular Weight: 614.88 (anhydrous basis)
MDL Number: MFCD00012116
Linear Formula: C₃₂H₅₈N₂O₇S · xH₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-C3023-1G	1 g	$61.80 1/EA	Add To Favorites
45-C3023-5G	5 g	$185.00 1/EA	Add To Favorites
45-C3023-25G	25 g	$746.00 1/EA	Add To Favorites
45-C3023-100G	100 g	$2040.00 1/EA	Add To Favorites
45-C3023-1KT	1 kit	$670.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: C3023-100G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
biological source	bovine bile
CMC	8 mM
description	zwitterionic
form	powder
grade	HPLC grade
impurities	<0.04% DMF
InChI	1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key	SJCUTFKCLFLIFE-JWTJKVBLSA-N
mol wt	614.88 g/mol (anhydrous basis)
mp	157 °C (dec.) (lit.)
Quality Level	400
shipped in	ambient
SMILES string	O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
solubility	water: 50 mg/mL
storage condition	dry at room temperature (tightly closed)
storage temp.	2-30°C
technique(s)	dialysis: suitable
	electrophoresis: suitable
transition temp	cloud point >100 °C

Application:	0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.
Features and Benefits:	• Suitable for Biochemical and Cell Biology research • Can be used in Dialysis and Electrophoresis • High purity product for research applications
General description:	CHAPS hydrate, a zwitterionic detergent derived from Cholic acid, stands out as an excellent tool in biochemical and cell biology research, particularly in the solubilization of membrane proteins. This versatile detergent, combining features of sulfobetaine-type and bile-acid detergents, exhibits a remarkable solubility rate of over 50% in aqueous solutions. It serves a pivotal role in stabilizing protein-DNA complexes, ensuring the retention of biochemical activity in solution. Widely utilized as a buffer in various research applications, CHAPS hydrate acts as an amphiphilic surfactant, proving effective in solubilizing plasma membrane proteins and diluting assay components. Notably, its easy removal through dialysis or gel filtration adds to its appeal, making it a preferred choice for molecular mass determination by fluorometry. This feature underscores CHAPS hydrate′s adaptability in diverse experimental setups. However, caution is advised in assays involving polyvalent metals or metal ions sensitive to nonspecific chelating agents. Beyond its conventional use, CHAPS hydrate finds application as a polymer matrix in binding assays, effectively removing metal ions from protein solutions.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1, 5, 25, 100 g in poly bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H360D
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
mp	157 °C (dec.) (lit.)
Storage Temp.	2-30°C
UNSPSC	12161900

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