CHAPS hydrate
SIAL/C3023 - ≥98% (HPLC)
Synonym: 3-
CAS Number: 75621-03-3 (anhydrous)
Empirical Formula (Hill Notation): C32H58N2O7S · xH2O
Molecular Weight: 614.88 (anhydrous basis)
Linear Formula: C32H58N2O7S · xH2O
Product Type: Chemical
assay | ≥98% (HPLC) |
CMC | 8 mM |
description | zwitterionic |
form | powder |
impurities | <0.04% DMF |
InChI | 1S/C32H58N2O7S.H2O/c1-21( |
InChI key | SJCUTFKCLFLIFE-JWTJKVBLSA |
mol wt | 614.88 g/mol (anhydrous basis) |
mp | 157 °C (dec.) (lit.) |
Quality Level | 400 ![]() |
SMILES string | O.C[C@H](CCC(=O)NCCC[N+]( |
technique(s) | dialysis: suitable |
electrophoresis: suitable | |
transition temp | cloud point >100 °C |
Application: | 0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS. |
General description: | A solution at 50 mg/ml in water yields a clear-colorless solution. Aqueous solutions of CHAPS can be autoclaved. |
General description: | CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis. |
Packaging: | 1, 5, 25, 100 g in poly bottle |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H360D |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC) |
mp | 157 °C (dec.) (lit.) |
UNSPSC | 12161900 |