5-Carboxyfluorescein diacetate
SIAL/C4916 - ~95% (HPLC)
Synonym: 5-CFDA; 5-Carboxy-di-O-acetylfluorescein
CAS Number: 79955-27-4
Empirical Formula (Hill Notation): C25H16O9
Molecular Weight: 460.39
MDL Number: MFCD00036872
Linear Formula: C25H16O9
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
assay | ~95% (HPLC) |
form | powder |
InChI | 1S/C25H16O9/c1-12(26)31-1 |
InChI key | WPUZGNPQMIWOHE-UHFFFAOYSA |
Quality Level | 200 ![]() |
SMILES string | CC(=O)Oc1ccc2c(Oc3cc(OC(C |
solubility | chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear |
storage temp. | −20°C |
Application: | 5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin. |
Biochem/physiol Actions: | 5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule. |
Packaging: | 100 mg in glass bottle |
Packaging: | 25 mg in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ~95% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12171500 |