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CHAPS hydrate

SIAL/C5070 - BioXtra, ≥98% (TLC)

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate; CHAPS

CAS Number: 75621-03-3 (anhydrous)
Empirical Formula (Hill Notation): C32H58N2O7S · xH2O
Molecular Weight: 614.88 (anhydrous basis)
Linear Formula: C32H58N2O7S · xH2O
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-C5070-1G 1 g
$118.00
1/EA
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45-C5070-5G 5 g
$401.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: C5070-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: C5070-1G

 

anion traces chloride (Cl-): ≤0.05%
assay ≥98% (TLC)
cation traces Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  Na: ≤0.005%
  NH4+: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
description zwitterionic
form powder
ign. residue ≤0.1%
impurities ≤0.001% Phosphorus (P)
  ≤0.1% Insoluble matter
  <0.04% DMF
InChI 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key SJCUTFKCLFLIFE-JWTJKVBLSA-N
mol wt 614.88 g/mol (anhydrous basis)
mp 157 °C (dec.) (lit.)
product line BioXtra
Quality Level 400 
SMILES string O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
solubility H2O: 0.1 M, clear, colorless
technique(s) protein purification: suitable
  protein quantification: suitable
Application: • 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.
General description: CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Packaging: 1, 5 g in poly bottle
Purity ≥98% (TLC)
mp 157 °C (dec.) (lit.)
UNSPSC 12161900

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