CHAPS hydrate
SIAL/C5070 - BioXtra, ≥98% (TLC)
Synonym: 3-
CAS Number: 331717-45-4
Empirical Formula (Hill Notation): C32H58N2O7S · xH2O
Molecular Weight: 614.88 (anhydrous basis)
MDL Number: MFCD00012116
Linear Formula: C32H58N2O7S · xH2O
Product Type: Chemical
anion traces | chloride (Cl-): ≤0.05% |
assay | ≥98% (TLC) |
cation traces | Al: ≤0.0005% |
Ca: ≤0.0005% | |
Cu: ≤0.0005% | |
Fe: ≤0.0005% | |
K: ≤0.005% | |
Mg: ≤0.0005% | |
Na: ≤0.005% | |
NH4+: ≤0.05% | |
Pb: ≤0.001% | |
Zn: ≤0.0005% | |
description | zwitterionic |
form | powder |
ign. residue | ≤0.1% |
impurities | ≤0.001% Phosphorus (P) |
≤0.1% Insoluble matter | |
<0.04% DMF | |
InChI | 1S/C32H58N2O7S.H2O/c1-21( |
InChI key | SJCUTFKCLFLIFE-JWTJKVBLSA |
mol wt | 614.88 g/mol (anhydrous basis) |
mp | 157 °C (dec.) (lit.) |
product line | BioXtra |
Quality Level | 400 |
SMILES string | O.C[C@H](CCC(=O)NCCC[N+]( |
solubility | H2O: 0.1 M, clear, colorless |
technique(s) | protein purification: suitable |
protein quantification: suitable |
Application: | • 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification. • 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA. • HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS. |
General description: | CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis. |
Packaging: | 1, 5 g in poly bottle |
Symbol | GHS08 |
Signal word | Danger |
Hazard statements | H360D |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 61-36/37/38 |
Safety Statements | 53-26-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (TLC) |
mp | 157 °C (dec.) (lit.) |
UNSPSC | 12161900 |