DL-Dithiothreitol

SIAL/D0632 - ≥98% (HPLC), ≥99.0% (titration)

Synonym: (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol; threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
Molecular Weight: 154.25
EC Number: 222-468-7
MDL Number: MFCD00004877
Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-D0632-1G	1 g	$66.80 1/EA	Add To Favorites
45-D0632-5G	5 g	DISCONTINUED
45-D0632-10G	10 g	$319.00 1/EA	Add To Favorites
45-D0632-25G	25 g	$476.11 1/EA	Add To Favorites
45-D0632-100G	100 g	$1530.00 1/EA	Add To Favorites
45-D0632-1KG	1 kg	$10710.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: D0632-25G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	general analytical
assay	≥98% (HPLC)
	≥99.0% (titration)
color	white
form	powder
InChI	1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key	VHJLVAABSRFDPM-QWWZWVQMSA-N
mp	41-44 °C (lit.)
Quality Level	200
reaction suitability	reagent type: reductant
SMILES string	O[C@H](CS)[C@H](O)CS
solubility	H₂O: soluble 50 mg/mL, clear, colorless to very faintly yellow
storage temp.	2-8°C

Application:	An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Application:	DTT has been used:• as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk. • as a component of medium for the demembranation and reactivation of spermatozoa. • to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst. • as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups. • in proteomics analysis as in-solution protein digestion for mass spectrometry • as a buffer component for protein quantification, to prepare wash buffer, lysis buffer, sample buffer, and protein elution buffer
Biochem/physiol Actions:	Dithiothreitol (DTT) is extensively applied in chemical peptide synthesis and biochemical preparations of thiol proteins. It is also used in protein chemistry studies, such as protein folding and enzyme activity. It also acts as an enzyme stabilizer to stop DNA modification. DTT specifically mediates the thiol-disulfide interchange reaction to entirely reduce the intra- or inter-molecular disulfide bonds in biomolecules. This reaction results in the formation of thiols and the cyclic disulfide of DTT.
Features and Benefits:	• High-quality DTT (HPLC≥98%), (titration≥99.0%) • Suitable for electrophoresis, proteomics analysis
General description:	Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than βME, hence efficient in lower concentrations.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly drum
Packaging:	1, 5, 10, 25, 100 g in poly bottle

Symbol	GHS05,GHS07
Signal word	Danger
Hazard statements	H302 - H315 - H318
Precautionary statements	P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Hazard Codes	Xn
Risk Statements	22-36/37/38
Safety Statements	26-36
RIDADR	UN 3335
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC); ≥99.0% (titration)
mp	41-44 °C (lit.)
Storage Temp.	2-8°C
UNSPSC	12352201

To Order From a Quote