DL-Dithiothreitol
SIAL/D0632 - ≥98% (HPLC), ≥99.0% (titration)
Synonym: threo-
CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C4H10O2S2
Molecular Weight: 154.25
EC Number: 222-468-7
MDL Number: MFCD00004877
Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
Product Type: Chemical
assay | ≥98% (HPLC) |
≥99.0% (titration) | |
color | white |
form | powder |
InChI | 1S/C4H10O2S2/c5-3(1-7)4(6 |
InChI key | VHJLVAABSRFDPM-QWWZWVQMSA |
mp | 41-44 °C (lit.) |
Quality Level | 200 ![]() |
reaction suitability | reagent type: reductant |
SMILES string | O[C@H](CS)[C@H](O)CS |
solubility | H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow |
storage temp. | 2-8°C |
Application: | An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystami |
Application: | DTT has been used: • as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk. • as a component of medium for the demembranation and reactivation of spermatozoa. • to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst. • as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups. |
General description: | An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystami |
Packaging: | 1 kg in poly drum |
Packaging: | 1, 5, 10, 25, 100 g in poly bottle |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H302 - H315 - H318 |
Precautionary statements | P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 22-36/37/38 |
Safety Statements | 26-36 |
RIDADR | UN 3335 |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥99.0% (titration) |
mp | 41-44 °C (lit.) |
Storage Temp. | 2-8°C |
UNSPSC | 12352201 |