Dihydrorhodamine 123
SIAL/D1054 - ≥95%
Synonym: DHR 123; Methyl 2-
CAS Number: 109244-58-8
Empirical Formula (Hill Notation): C21H18N2O3
Molecular Weight: 346.38
MDL Number: MFCD00285712
Linear Formula: C21H18N2O3
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥95% |
| color | pink |
| form | powder |
| InChI | 1S/C21H18N2O3/c1-25-21(24 |
| InChI key | FNEZBBILNYNQGC-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | COC(=O)c1ccccc1C2c3ccc(N) |
| solubility | DMSO: >5 mg/mL |
| storage temp. | −20°C |
| Application: | Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils. |
| Biochem/physiol Actions: | Dihydrorhodamine 123 detects superoxide ions at the single-cell level and is used for probing neutrophils superoxide generation in a respiratory burst event. It is also used for measuring oxidative stress in macrophages and human spermatozoa. |
| Biochem/physiol Actions: | Dihydrorhodamine 123 is used for the detection of ROS (reactive oxygen species). Presence of ROS oxidizes dihydrorhodamine 123 to the fluorescent derivative rhodamine 123. The reaction happens in the presence of a peroxidase or a similar catalyst. |
| General description: | Dihydrorhodamine 123 (DHR123) is an uncharged non-fluorescent dye and a derivative of rhodamine 123 (R123). Dihydrorhodamine 123 (DHR123) is oxidized to fluorescent R123 within cells in the presence of reactive oxygen species and it localizes in mitochondria. It is an analog of 2,7-Dichlorodihydrofluore |
| Packaging: | 2, 10 mg in ampule |
| Substrates: | Peroxidase substrate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |