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DL-Dithiothreitol

SIAL/D5545 - BioXtra, ≥99.0% (titration)

Synonym: threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C4H10O2S2
Molecular Weight: 154.25
EC Number: 222-468-7
MDL Number: MFCD00004877
Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-D5545-1G 1 g
$90.00
1/EA
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45-D5545-5G 5 g
$286.00
1/EA
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45-D5545-25G 25 g
$1050.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5545-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5545-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5545-5G

 

absorption ≤0.100 at 280 in H2O at 0.1 M
  ≤0.400 at 260 in H2O at 0.1 M
anion traces sulfate (SO42-): ≤0.005%
assay ≥99.0% (titration)
cation traces Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.1%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
form powder
impurities Insoluble matter, passes filter test
  ≤0.3% 4,5-dihydroxy-1,2-dithiane
InChI 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key VHJLVAABSRFDPM-QWWZWVQMSA-N
mp 41-44 °C (lit.)
pH 4.0-6.0 (20-25 °C, 0.1 m in H2O)
product line BioXtra
Quality Level 200 
reaction suitability reagent type: reductant
SMILES string O[C@H](CS)[C@H](O)CS
solubility H2O: 0.1 M, clear, colorless
storage temp. 2-8°C
Application: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Packaging: 1, 5, 25 g in glass bottle
Purity ≥99.0% (titration)
mp 41-44 °C (lit.)
Storage Temp. 2-8°C
UNSPSC 12352201

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