3,3′-Diaminobenzidine
SIAL/D8001 - ≥97% (HPLC)
Synonym: 3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl; DAB
CAS Number: 91-95-2
Empirical Formula (Hill Notation): C12H14N4
Molecular Weight: 214.27
EC Number: 202-110-6
MDL Number: MFCD00007725
Linear Formula: (NH2)2C6H3C6H3(NH2)2
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
assay | ≥97% (HPLC) |
form | powder |
InChI | 1S/C12H14N4/c13-9-3-1-7(5 |
InChI key | HSTOKWSFWGCZMH-UHFFFAOYSA |
mp | 175-177 °C (lit.) |
Quality Level | 200 ![]() |
SMILES string | NC1=C(N)C=CC(C2=CC(N)=C(N |
storage temp. | room temp |
Application: | 3,3′-Diaminobenzidine has been used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections. |
Application: | Peroxidase substrate; reagent for spectrophotometric determination of selenium. |
Biochem/physiol Actions: | DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures. |
Packaging: | 1, 5 g in glass bottle |
Packaging: | 10, 25 g in poly bottle |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H302 - H319 - H341 - H350 |
Precautionary statements | P201 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | T |
Risk Statements | 45-68 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥97% (HPLC) |
mp | 175-177 °C (lit.) |
Storage Temp. | room temp |
UNSPSC | 12171500 |