3,3′-Diaminobenzidine
SIAL/D8001 - 97% (HPLC)
Synonym: 3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl; DAB
CAS Number: 91-95-2
Empirical Formula (Hill Notation): C12H14N4
Molecular Weight: 214.27
EC Number: 202-110-6
MDL Number: MFCD00007725
Linear Formula: (NH2)2C6H3C6H3(NH2)2
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | 97% (HPLC) |
| form | powder |
| InChI | 1S/C12H14N4/c13-9-3-1-7(5 |
| InChI key | HSTOKWSFWGCZMH-UHFFFAOYSA |
| mp | 175-177 °C (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | NC1=C(N)C=CC(C2=CC(N)=C(N |
| storage temp. | room temp |
| Application: | 3,3′-Diaminobenzidine has been used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections. |
| Application: | Peroxidase substrate; reagent for spectrophotometric determination of selenium. |
| Biochem/physiol Actions: | DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research ![]() |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 10, 25 g in poly bottle |
| Symbol | ![]() GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H319 - H341 - H350 |
| Precautionary statements | P201 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 45-68 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 97% (HPLC) |
| mp | 175-177 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |



