3,3′-Diaminobenzidine tetrahydrochloride hydrate
SIAL/D9015 - ISOPAC®
CAS Number: 868272-85-9
EC Number: 231-018-9
MDL Number: MFCD08273058
Linear Formula: C12H14N4 · 4HCl · xH2O
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
assay | ≥96% (TLC) |
form | powder |
InChI | 1S/C12H14N4.4ClH.H2O/c13- |
InChI key | DXWSCXIZIHILNP-UHFFFAOYSA |
mp | 280 °C |
Quality Level | 100 |
SMILES string | O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N |
solubility | water: 50 mg/mL, clear to slightly hazy |
storage temp. | room temp |
Application: | 3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization. |
Biochem/physiol Actions: | DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures. |
Caution: | Injecting a compatible solvent permits preparation of any desired strength solution without exposure. |
Legal Information: | Isopac is a registered trademark of Merck KGaA, Darmstadt, Germany |
Packaging: | 100 mg in serum bottle |
Packaging: | Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal. |
Reconstitution: | Dissolving the contents in 100 mL of solvent yields a 0.1% solution. |
Symbol | GHS08 |
Signal word | Danger |
Hazard statements | H341 - H350 |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 45-68 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Purity | ≥96% (TLC) |
mp | 280 °C |
Storage Temp. | room temp |
UNSPSC | 12171500 |