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3,3′-Diaminobenzidine tetrahydrochloride hydrate

SIAL/D9015 - ISOPAC®

CAS Number: 868272-85-9
Empirical Formula (Hill Notation): C12H14N4 · 4HCl · xH2O
Molecular Weight: 360.11 (anhydrous basis)
EC Number: 231-018-9
MDL Number: MFCD08273058
Linear Formula: C12H14N4 · 4HCl · xH2O
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-D9015-100MG 100 mg
$98.20
1/EA
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application(s) diagnostic assay manufacturing
hematology
histology
assay ≥96% (TLC)
form powder
InChI 1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key DXWSCXIZIHILNP-UHFFFAOYSA-N
mp 280 °C
Quality Level 100 
SMILES string O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
solubility water: 50 mg/mL, clear to slightly hazy
storage temp. room temp
Application: 3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.
Biochem/physiol Actions: DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.
Caution: Injecting a compatible solvent permits preparation of any desired strength solution without exposure.
Legal Information: Isopac is a registered trademark of Sigma-Aldrich Co. LLC
Packaging: 100 mg in serum bottle
Packaging: Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.
Reconstitution: Dissolving the contents in 100 mL of solvent yields a 0.1% solution.
Purity ≥96% (TLC)
mp 280 °C
Storage Temp. room temp
UNSPSC 12171500

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