DAPI, dilactate
SIAL/D9564 - ≥98% (HPLC)
Synonym: 4′,6-Diamidino-2-phenyindole, dilactate
Empirical Formula (Hill Notation): C16H15N5 · 2C3H6O3
Molecular Weight: 457.48
MDL Number: MFCD00467366
Linear Formula: C16H15N5 · 2C3H6O3
Product Type: Chemical
Immunofluorescence A. NRK cells were fixed, permeabilized with Methanol followed by Acetone and stained with 1 μg/mL DAPI (Cat. No. D9564) (blue). 40× magnification. B. NRK cells were fixed, permeabilized with Methanol followed by Acetone and golgi were stained with 5 μg/mL Anti-TGN38 antibody produced in Rabbit (Cat. No. T9826) followed by Anti-Rabbit IgG-FITC (Cat No. F9887). Cells were counterstained with 1 μg/mL DAPI (Cat. No. D9564) (blue). 40× magnification.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D9564-10MG
| ε (extinction coefficient) | 28.00-32.50 at 345-351 nm in methanol |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥98% (HPLC) |
| color | yellow |
| form | powder |
| InChI | InChI=1S/C16H15N5.C3H6O3/ |
| InChI key | LCAUPANIXBMNIA-UHFFFAOYSA |
| Quality Level | 300  |
| SMILES string | CC(O)C(O)=O.CC(O)C(O)=O.N |
| solubility | H2O: 20 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | titration: suitable |
| Application: | DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures. |
| Biochem/physiol Actions: | A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts. |
| General description: | DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |