Fast Blue B Salt
SIAL/D9805 - Dye content ~95 %
Synonym: o-Dianisidine bis(diazotized) zinc double salt; Azoic Diazo No. 48; DBB; Diazo Blue B; Naphthanil Diazo Blue B
CAS Number: 14263-94-6
Empirical Formula (Hill Notation): C14H12Cl4N4O2Zn
Molecular Weight: 475.47
EC Number: 238-153-2
MDL Number: MFCD00075203
Linear Formula: C14H12N4O2Cl2 · ZnCl2
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
composition | Dye content, ~95% |
description | Technical grade |
form | powder |
InChI | 1S/C14H12N4O2.4ClH.Zn/c1- |
InChI key | GPPKNJIWDULNQH-UHFFFAOYSA |
mp | >300 °C (lit.) |
Quality Level | 200 ![]() |
SMILES string | [Cl-].[Cl-].Cl[Zn]Cl.COc1 |
solubility | H2O: 1 mg/mL |
storage temp. | 2-8°C |
Application: | • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens. • It has been used as a spraying agent in thin layer chromatography for the identification of separated components. • It has also been used for the determination of the lipase activity. |
Biochem/physiol Actions: | Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity. |
Packaging: | 10, 100 g in glass bottle |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H242 - H350 |
Precautionary statements | P202 - P210 - P235 - P308 + P313 - P370 + P378 - P403 |
Hazard Codes | T |
Risk Statements | 45 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | >300 °C (lit.) |
Storage Temp. | 2-8°C |
Colour Index Number | 37235 |
UNSPSC | 12171500 |