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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

SIAL/E1769 - BioXtra

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC; EDC hydrochloride; WSC hydrochloride

CAS Number: 25952-53-8
Empirical Formula (Hill Notation): C8H17N3 · HCl
Molecular Weight: 191.70
EC Number: 247-361-2
MDL Number: MFCD00012503
Linear Formula: C8H17N3 · HCl
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-E1769-1G 1 g
$83.30
1/EA
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45-E1769-5G 5 g
$202.00
1/EA
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45-E1769-10G 10 g
$346.00
1/EA
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45-E1769-25G 25 g
$687.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: E1769-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: E1769-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: E1769-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: E1769-10G

 

anion traces sulfate (SO42-): ≤0.05%
cation traces Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  Na: ≤0.005%
  NH4+: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
form powder
ign. residue ≤0.1%
impurities ≤0.005% Phosphorus (P)
  ≤0.1% Insoluble matter
InChI 1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key FPQQSJJWHUJYPU-UHFFFAOYSA-N
mp 110-115 °C (lit.)
product line BioXtra
Quality Level 200 
SMILES string Cl.CCN=C=NCCCN(C)C
solubility H2O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow
storage temp. −20°C
Application: N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
• for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
• for cross-linking of nanofiber matrices with mouse lung extract
• for activation of substrate XNA oligonucleotides

Biochem/physiol Actions: Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.
Packaging: 1, 5, 10, 25 g in poly bottle
mp 110-115 °C (lit.)
Storage Temp. −20°C
UNSPSC 12161700

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