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Ethidium homodimer I solution

SIAL/E1903 - ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO

Synonym: EtDi; EthD-1; Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride

Empirical Formula (Hill Notation): C46H50Cl4N8
Molecular Weight: 856.75
EC Number: 200-664-3
MDL Number: MFCD00210948
Linear Formula: C46H50Cl4N8
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-E1903-.5ML 0.5 mL
 $439.00
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ε (extinction coefficient) ≥9000 at 490-496 nm in water
application(s) diagnostic assay manufacturing
hematology
histology
assay ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
color red
concentration 2 mM in DMSO
form liquid
InChI 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
InChI key ZKAJUKABUUJRLB-UHFFFAOYSA-N
Quality Level 100 
SMILES string Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
storage temp. −20°C
technique(s) titration: suitable
Application: Ethidium homodimer I solution has been used to check membrane integrity in sperms.
Application: Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.
Application: Used to detect DNA in solution.
Biochem/physiol Actions: Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.
Biochem/physiol Actions: One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.
RIDADR NA 1993 / PGIII
WGK Germany WGK 1
Flash Point(F) 185.0 °F - closed cup
Flash Point(C) 85 °C - closed cup
Purity ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
Storage Temp. −20°C
UNSPSC 12171500

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