Ethylenediaminetetraacetic acid disodium salt dihydrate

SIAL/E4884 - ACS reagent, 99.0-101.0%

Synonym: Disodium ethylenediaminetetraacetate dihydrate; EDTA disodium salt; EDTA-Na₂; Edathamil; Edetate disodium salt dihydrate; Sequestrene Na₂

CAS Number: 6381-92-6
Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
Molecular Weight: 372.24
EC Number: 205-358-3
Linear Formula: C₁₀H₁₄N₂Na₂O₈ · 2H₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E4884-100G	100 g	$59.20 1/EA	Add To Favorites
45-E4884-500G	500 g	$166.00 1/EA	Add To Favorites

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This image depicts SKU: E4884-100G

Properties
Descriptions
Safety Info.
Basic Data

agency	suitable for SM 4500 - NH3
application(s)	microbiology
assay	99.0-101.0%
cation traces	Fe: ≤0.01%
	heavy metals: ≤0.005% (by ICP-OES)
form	solid
grade	ACS reagent
impurities	≤0.005% Insoluble matter
	≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
InChI	1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;21H2/q;2+1;;/p-2
InChI key	OVBJJZOQPCKUOR-UHFFFAOYSA-L
mp	248 °C (dec.) (lit.)
pH	4.0-6.0 (25 °C, 5%)
Quality Level	200
reaction suitability	reagent type: chelator
SMILES string	O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
solubility	H₂O: 100 g/L at 20 °C
storage temp.	room temp

Application:	Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.
Application:	Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Application:	Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component: • of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate • of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs) • of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart
Features and Benefits:	American Chemical Society (ACS) grade is suitable for use in standard analytical methods
General description:	Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	100, 500 g in poly bottle

Symbol	GHS07,GHS08
Signal word	Warning
Hazard statements	H332 - H373 - H412
Precautionary statements	P260 - P271 - P273 - P304 + P340 + P312 - P314 - P501
Hazard Codes	Xn
Risk Statements	20-48/20
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	99.0-101.0%
mp	248 °C (dec.) (lit.)
Storage Temp.	room temp
UNSPSC	12352107

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