N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
SIAL/E6383 - crystalline
Synonym: EDC; N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC; EDC hydrochloride; WSC hydrochloride
CAS Number: 25952-53-8
Empirical Formula (Hill Notation): C8H17N3 · HCl
Molecular Weight: 191.70
EC Number: 247-361-2
MDL Number: MFCD00012503
Linear Formula: C8H17N3 · HCl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E6383-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E6383-5G
| application(s) | advanced drug delivery general analytical |
| color | white to off-white |
| form | crystalline |
| InChI | 1S/C8H17N3.ClH/c1-4-9-8-1 |
| InChI key | FPQQSJJWHUJYPU-UHFFFAOYSA |
| mp | 110-115 °C (lit.) |
| Quality Level | 200  |
| reaction suitability | reagent type: cross-linking reagent reaction type: Peptide Synthesis |
| SMILES string | Cl.CCN=C=NCCCN(C)C |
| solubility | H2O: ≤100 mg/mL |
| storage temp. | −20°C |
| Application: | N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used: • for the immobilisation of trypsin onto self-assembled monolayers (SAMs) • as a component for the preparation of collagen matrices • for the preparation of phosphoethanolamine(PEt)- |
| Biochem/physiol Actions: | Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided. |
| Features and Benefits: | Versatile and adaptable for a wide variety of laboratory and research applications |
| General description: | 1-Ethyl-3-(3-dimethylamin Beyond peptides, EDC HCl extends its influence to the construction of immunogens, where it covalently attaches haptens (small immune-response eliciting molecules) to carrier proteins, playing an instrumental role in vaccine research. The versatility of EDAC HCl further unfolds in its ability to modify nucleic acids, allowing for the labeling of DNA and RNA through their 5′ phosphate groups. This facilitates the visualization, tracking, and analysis of these essential molecules, contributing to advancements in nucleic acid research. Additionally, EDAC HCl serves as a biomolecule bridge by acting as a crosslinker, connecting amine-reactive NHS-esters of biomolecules to carboxyl groups. This technique proves valuable in protein conjugation, enabling the creation of hybrid molecules with novel properties and functions. The underlying mechanism of EDAC HCl involves its reaction with a carboxyl group, forming an unstable intermediate that actively seeks an amine partner. The delicate balance of this reaction underscores the importance of optimizing conditions for efficient conjugation. The assistance of N-hydroxysuccinimide (NHS) further enhances EDAC HCl′s capabilities, stabilizing the intermediate and enabling two-step conjugation procedures. This additional feature provides greater flexibility and control, particularly in dealing with complex biomolecules. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 1, 5 g in glass bottle |
| Symbol |    GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H311 - H315 - H317 - H373 - H410 |
| Precautionary statements | P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314 |
| Hazard Codes | Xi |
| Risk Statements | 37/38-41 |
| Safety Statements | 26-36/37/39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 110-115 °C (lit.) |
| Storage Temp. | −20°C |
| UNSPSC | 12161703 |