N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

SIAL/E7750 - commercial grade, powder

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC; EDC hydrochloride; WSC hydrochloride

CAS Number: 25952-53-8
Empirical Formula (Hill Notation): C₈H₁₇N₃ · HCl
Molecular Weight: 191.70
EC Number: 247-361-2
MDL Number: MFCD00012503
Linear Formula: C₈H₁₇N₃ · HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E7750-100MG	100 mg	$25.70 1/EA	Add To Favorites
45-E7750-1G	1 g	$31.00 1/EA	Add To Favorites
45-E7750-5G	5 g	$103.00 1/EA	Add To Favorites
45-E7750-10G	10 g	$172.00 1/EA	Add To Favorites
45-E7750-25G	25 g	$339.00 1/EA	Add To Favorites
45-E7750-100G	100 g	$1180.00 1/EA	Add To Favorites
45-E7750-1KG	1 kg	$5370.00 1/EA	Add To Favorites
45-E7750-5KG	5 kg	$19020.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: E7750-10G

Properties
Descriptions
Safety Info.
Basic Data

color	white to off-white
form	powder
grade	commercial grade
InChI	1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key	FPQQSJJWHUJYPU-UHFFFAOYSA-N
mp	110-115 °C (lit.)
Quality Level	300
SMILES string	Cl.CCN=C=NCCCN(C)C
solubility	H₂O: ≤100 mg/mL
storage temp.	−20°C
technique(s)	bioconjugation: suitable
	Northern blotting: suitable

Application:	• N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used for the formation of FND (fluorescent nanodiamonds)-transferrin bioconjugates. • It has been used for crosslinking polyethylenimine to gold particles. • It has been used as a carbodiimide linkage agent for coating of carboxylated polystyrene beads with biotinylated BSA (bovine serum albumin).
Biochem/physiol Actions:	Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.
Features and Benefits:	Versatile and adaptable for a wide variety of laboratory and research applications
General description:	1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC HCl), commonly referred to as EDAC, is a highly potent and extensively utilized water-soluble reagent in the realm of chemical and biochemical research, playing a crucial role in facilitating the formation of amide bonds. Within the domain of peptide synthesis, EDC HCl demonstrates notable efficiency by catalyzing the coupling of amino acids through their carboxyl and amine groups, thereby facilitating the creation of peptide backbones with specific sequences and functionalities. This particular attribute is highly valuable in the production of peptides for various research purposes. Expanding beyond peptides, EDC HCl′s influence extends to the construction of immunogens, where it actively participates in the covalent attachment of haptens (small molecules that elicit an immune response) to carrier proteins. This involvement proves instrumental in the research and development of vaccines. The versatility of EDC HCl further manifests in its capacity to modify nucleic acids, allowing for the labeling of DNA and RNA through their 5′ phosphate groups. This functionality enhances the visualization, tracking, and analysis of these crucial molecules, contributing significantly to the progression of nucleic acid research. Moreover, EDC HCl serves as a vital biomolecule bridge, acting as a crosslinker that connects amine-reactive NHS-esters of biomolecules to carboxyl groups. This technique is particularly valuable in protein conjugation, enabling the creation of hybrid molecules with novel properties and functions. The underlying mechanism of EDC HCl involves its reaction with a carboxyl group, forming an unstable intermediate that actively seeks an amine partner. The delicate balance of this reaction emphasizes the need for optimizing conditions to ensure efficient conjugation. The assistance of N-hydroxysuccinimide (NHS) further enhances EDC HCl′s capabilities by stabilizing the intermediate and enabling two-step conjugation procedures, offering greater flexibility and control, especially when dealing with complex biomolecules.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1, 5 kg in poly drum
Packaging:	1, 5, 10, 25, 100 g in poly bottle
Packaging:	100 mg in poly bottle

Symbol	GHS06,GHS08,GHS09
Signal word	Danger
Hazard statements	H302 - H311 - H315 - H317 - H373 - H410
Precautionary statements	P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314
Hazard Codes	Xi
Risk Statements	37/38-41
Safety Statements	26-36/37/39
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

mp	110-115 °C (lit.)
Storage Temp.	−20°C
UNSPSC	12352111

To Order From a Quote